Russian Federation
Variations in the concentration and photosynthesis of aquatic biota are important components of the processes that determine the vital functions of aquatic biota, the carbon dioxide content in Lake Baikal water, and, consequently, its fluxes in the water-atmosphere system. Laboratory methods for determining algal chlorophyll concentrations are insufficiently time-consuming and labor-intensive, limiting the collection of detailed information on their distribution throughout the lake. Using several cycles of shipboard measurements as an example, this paper demonstrates our method for stepwise calibration of the results of synchronous measurements of fluorescence characteristics using flow-through and laboratory fluorimeters and data from a classical laboratory method for determining chlorophyll-a concentration.
CHLOROPHYLL, SURFACE WATER, LAKE BAIKAL
1. Zavoruev V.V., Domysheva V.M., Shimaraev M.N., Sakirko M.V., Pestunov D.A., Panchenko M.V. Prostranstvennoe raspredelenie fluorescentnyh harakteristik fitoplanktona v period formirovaniya vesenney gomotermii v oz. Baykal. // Optika atmosfery i okeana. 2008. T. 21. № 5. S. 377-380.
2. Domysheva V. M., Usol'ceva M. V., Sakirko M. V., Pestunov D. A., Shimaraev M. N., Popovskaya G. I., Panchenko M. V. Prostranstvennoe raspredelenie potokov uglekislogo gaza, biogennyh elementov i biomassy fitoplanktona v pelagiali oz. Baykal v vesenniy period 2010–2012 gg. . // Optika atmosfery i okeana. 2014. T. 27. № 06. S. 539–545.
3. Bondarenko N. A., Rusanov I. I., Chernicyna S. M. i dr. Struktura i produkcionnyy potencial letnego fitoplanktona ozera Baykal v sovremennyy period // Vodnye resursy. — 2022. — T. 49, № 1. — S. 66—76. — https://doi.org/10.31857/s0321059622010059.
4. Beer S, Ilan M, Eshel A, Weil A and Brickner L (1998) Use of pulse amplitude modulated (PAM) fluorometry for in situ measurements of photosynthesis Red Sea faviid coarals Mar Biol 131: 697-612
5. Schreiber U, Bilger W, Neubauer C. 1995. Chlorophyll fluorescence as a nonintrusive indicator For rapid assessment of in vivo photosynthesis. In: Schultze E-O, Caldwell
6. Lorenzen C.J. A method for the continuous measurement of in vivo chlorophyll concentration // Deep-Sea Res. Oxford, 1966. Vol. 13. P. 223–227.
7. Karabashev G.S. Flyuorescenciya v okeane. L.: Gidrometeoizdat, 1987. 200 s.
8. Ostrowska M. Fluorescence “in situ” method for the determination of chlorophyll a concentration in sea // Oceanologia. 1990. Vol. 29. P. 175–202.
9. Sakirko M. V., Blinov V. V., Makarov M. M., Vorob'eva S. S., Fedotov A. P., i Domysheva V. M. Soderzhanie hlorofilla 𝑎 i ego svyaz' s biogennymi elementami v pelagiali ozera Baykal // Russian Journal of Earth Sciences. — 2026. — T. 26. — ES1005. — DOI:https://doi.org/10.2205/2026es001038 — EDN: https://elibrary.ru/TVVGEK
