Institut problem ekologii i evolyucii RAN
Russian Federation
UDC 535.37
UDC 57.044
CSCSTI 29.31
Russian Classification of Professions by Education 03.03.02
Russian Library and Bibliographic Classification 223
Russian Trade and Bibliographic Classification 6135
BISAC SCI053000 Physics / Optics & Light
The uncontrolled spread of antibiotics in wastewater and natural environments currently poses a serious environmental threat in all countries. Therefore, the development of rapid methods for detecting antibiotics in natural environments is extremely relevant. The work is devoted to the study of the spectral characteristics of three veterinary antibiotics (tylosin, tetracycline, ciprofloxacin) of different concentrations in the presence of organic substances of natural origin or by-products of animal husbandry. All three antibiotics have absorption bands in the UV region, which were isolated by mathematical processing against the background of a wide absorption spectrum of organic matter in water, soil or organic fertilizers, monotonically decreasing with increasing wavelength. The antibiotic concentration was determined by the area of the absorption band or absorbance value at a fixed wavelength using relevant calibration dependencies. Fluorescence spectroscopy was also used to monitor low concentrations of ciprofloxacin, less than 5 mg/l. The emission band of ciprofloxacin partially overlaps with the fluorescence band of organic matter in water and soil, so to isolate the contribution of this antibiotic to the spectra, synchronous fluorescence spectra with a constant difference in excitation and recording wavelengths were measured. In this case, the antibiotic concentration was determined by the area of the synchronous fluorescence spectrum band in the UV range, in the region of the absorption band of this compound.
veterinary antibiotics, monitoring, absorption spectra, fluorescence.
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